Alpha-adducin is a protein encoded by the ADD1 gene.
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma).
Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure.
Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Alpha- and gamma-adducins are ubiquitously expressed.
In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues.
Adducin binds with high affinity to Ca(2)/calmodulin and is a substrate for protein kinases A and C.
Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ADD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr99-Gln328 of human ADD1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
