84-4405-89 ADAMTS16 (A Disintegrin And Metalloproteinase With Thrombospondin 16, ADAM Metallopeptidase With Thrombospondin Type 1 Motif 16) 200ul 362002
84-4405-89 ADAMTS16 (A Disintegrin And Metalloproteinase With Thrombospondin 16, ADAM Metallopeptidase With Thrombospondin Type 1 Motif 16) 200ul 362002
ADAMTS16 is a member of the large ADAMTS family of zinc-dependent proteases.
The deduced protein contains an N-terminal signal peptide, followed by a propeptide; a metalloprotease catalytic domain that includes the Zn-binding motif; a disintegrin-like domain; a central thrombospondin I (THBS1)-like motif; a cysteine-rich domain; a spacer region; and 3 C-terminal THBS1 repeats.
It also has 7 N-glycosylation sites.
ADAMTS16 shares 57% sequence identity with ADAMTS18, including 85% identity within the catalytic domains.
PCR analysis revealed that ADAMTS16 is expressed in fetal lung and kidney and in adult prostate and ovary.Rat Adamts16, which encodes a 1221aa protein, and they detected prominent expression in rat kidney as well as thymus, lung, heart, liver and spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human ADAMTS16.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Leu290-Pro641 of human ADAMTS16, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.