84-4405-87　ADAMTS12 (A Disintegrin And Metalloproteinase With Thrombospondin 12) 200ul　362000

特徴

• ADAMTS12 possesses all the domains characteristic of ADAMTSs, including signal sequence, propeptide, metalloproteinase-like, disintegrin-like, and cysteine-rich domains, as well as a series of 8 uniquely organized thrombospondin I (THBS1) repeats.
• Expression was upregulated by transforming growth factor-beta (TGFB) in fetal fibroblasts.
• RT-PCR analysis detected wide expression of ADAMTS12 in gastric carcinomas but not in adjacent normal tissues.
• ADAMTS12 is expressed as proteins of 83 and 120kD, considerably lower masses than the 17kD protein expressed in vitro.
• ADAMTS12 is extensively processed at its N-terminal end, after the cysteine-rich domain and around the first THBS1 domain, by a metalloprotease in cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGHHHHHHSG SEF- QQMYFWQY GHWTECSVTC GTGIRRQTAH CIKKGRGMVK ATFCDPETQP NGRQKKCHEK ACPPRWWAGE WEACSATCGP HGEKKRTVLC IQTMVSDEQA LPPTDCQHLL KPKTLLSCNR DILCPSDWTV GNWSECSVSC GGGVRIRSVT CAKNHDEPCD VTRKPNSRAL CGLQQCP
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.