Actinin, alpha 3, also known as ACTN3, is a protein which in humans is encoded by the ACTN3 gene.
Alpha-actinin is an actin-binding protein with multiple roles in different cell types.
This gene expression is limited to skeletal muscle.
It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments
Skeletal muscle is composed of long cylindrical cells called muscle fibers.
There are two types of muscle fibers, slow twitch or muscle contraction (type I) and fast twitch (type II).
Slow twitch fibers are more efficient in using oxygen to generate energy whilst fast twitch fibers are less efficient.
However, fast twitch fibers fire more rapidly and generate more force.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ACTN3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu162-Lys416 of human ACTN3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
