Actin, aortic smooth muscle is one of six different actin isoforms which have been identified.
Six different actin isoforms have been identified in vertebrates by amino acid sequencing: skeletal muscle, cardiac muscle, 2 smooth muscle (enteric and aortic), and 2 cytoplasmic (beta and gamma) (Vandekerckhove and Weber, 1979).
Their amino acid sequences are similar and well conserved in evolution; e.g., skeletal and cardiac actins differ by only 4aa, and skeletal muscle and cytoplasmic beta-actins differ by only 25aa out of a total of 374aa
Ueyama et al.
(1984) isolated and characterized the human aortic smooth muscle actin gene.
The gene has a transition point mutation in position 309, substituting thymine for cytosine.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ACTa2. Species Crossreactivity: equine, chicken, mouse, rat, guinea pig, bovine, monkey and rabbit
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo Ch Eq Gp Mk Mo Rb Rt
Immunogen：Recombinant protein corresponding to Ile167-Phe377 of human ACTa2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
