ACP5 is a glycosylated monomeric metalloenzyme expressed in mammals.
It has a molecular weight of ~35kD, a basic isoelectric point (7.6-9.5), and optimal activity in acidic conditions.
TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction.
It is differentiated by other mammalian acid phosphatases by its resistance to inhibition by tartrate, molecular weights and characteristic purple color.
The mechanism of phosphate ester hydrolysis by TRAP is through a nucleophilic attack mechanism, whereby, catalysis occurs with the binding of a phosphate-substrate to the Fe2+ in the active site of TRAP.
This is then followed by a nucleophilic attack by a hydroxide ligand on the bound phosphorus atom, resulting in cleavage of the phosphate ester bond and production of an alcohol.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
