ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase.
This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human.
These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
As the human TCP1 gene had been assigned to 6q25-q27 by study of somatic cell hybrids and by in situ hybridization, the ACAT2 gene was suspected to be localized to the same chromosome region.
Retention of this close linkage during mammalian evolution suggests the possibility of some functional significance.
Transcription of both DNA strands at a given locus is common in prokaryotic and viral systems.
For examples of overlapping transcriptional units in humans.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ACAT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Gly397 of mouse ACAT2, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
