Members of the ABC family of membrane proteins, such as ABCC10, are involved in energy-dependent transport of a wide variety of substrates across membranes.
The 1,492aa ABCC10 protein has a calculated molecular mass of 162kD.
It contains 2 nucleotide-binding folds, 17 predicted transmembrane segments arranged in 3 transmembrane domains, and 3 predicted N-glycosylation sites.
Northern blot analysis was unable to detected ABCC10 expression, but RT-PCR detected ABCC10 expression in all 10 tissues examined.
In vitro-translated ABCC10 had an apparent molecular mass of 158kD by SDS-PAGE.
Basal MRP7 promoter activity relied upon a proximal segment of the 5-prime flanking region bearing an E2F site acting cooperatively with 2 closely positioned SP1 sites.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ABCC10.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val1246-Pro1492 of human ABCC10, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
