AATK is a novel gene which is induced during apoptosis.
It encodes a protein with a tyrosine kinase domain at the N-terminal end and a proline-rich domain at the C-terminal end.
AATK expression may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells.
By randomly selecting cDNA clones from size-fractionated human brain cDNA libraries, Ishikawa et al.
(1998) identified a full-length cDNA clone, which they called KIAA0641, encoding a protein with significant homology to the mouse Aatyk protein identified by Gaozza et al.
(1997).
The deduced human AATK protein contains 1207aa and has an observed molecular mass greater than 100kD.
By RT-PCR, Ishikawa et al.
(1998) detected expression of AATK in brain, lung, kidney, and pancreas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human AATK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu1216-Ala1374 of human AATK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
