84-4405-33　AATF (Apoptosis Antagonizing Transcription Factor, CHE1, DED, Rb-binding protein Che-1) 200ul　361946

特徴

• Protein AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis.
• This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain.
• Overexpression of this gene interfered with MAP3K12 induced apoptosis.
• By differential display for genes downregulated by TGFB in an intestinal crypt epithelial cell line, Lindfors et al.
• (2000) cloned AATF.
• The AATF transcript contains several 4-nucleotide repeats, and the deduced 560aa protein contains a leucine zipper motif, several phosphorylation sites, a nuclear localization signal, and 3 nuclear receptor LxxLL binding motifs.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-S ALKNSHKALK ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG FGAFERSILT QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL PGEPETLPQG PANAHLRDLD EEIFDDDDFY HQLLRELI
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.