The longest variant, AAT1-alpha, encodes a deduced 98aa protein.
The shorter transcripts, AAT1-beta and AAT1-gamma, encode identical proteins that correspond to the 31aa C-terminal of AAT1-alpha.
Western blot analysis of mouse testis detected endogenous Aat1-alpha at an apparent molecular mass of 10.8kD.
AAT1 was expressed in all mouse, rat, and human spermatogenesis-related tissues and cell lines examined.
Expression was also detected in all cell lines of non-testis origin examined.
In situ hybridization and immunohistochemical analysis of mouse testis detected Aat1 during all stages of spermatogenesis and in Leydig cells.
Aat1 was also detected in the neck region of mature sperm.
AAT1 colocalized with AMY1 in mitochondria in transfected human and mouse cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SH ISSNRAYDFL YDPLFIVSSE KDHTQANIQA TLIRSRLRKV PRFKTMFSNL IHYPRYSLYW SKSDPVPPFI SREWKGHKEK HREALRQLTT TDASFQMPKE VYEDPEVTGK NRYKYFERPF LPFFQQMPFN VVYAVSKAEP YTFPPTSTKH L
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human AAT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser29-Leu181 of human AAT1, expressed in E. coli.
