Lactosylceramide 4-alpha-galactosyltransferase is an enzyme catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system.
The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor.
The A4GALT gene encodes alpha-1,4-galactosyltransferase, an enzyme that transfers a galactose to the alpha-1,4 position of lactosylceramide to form globotriaosylceramide (GB3), also known as CD77.
This glycolipid has been characterized on red blood cells as the P(k) antigen of the P blood group system.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes mouse a4GALT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa94-270 of mouse a4GALT, expressed in E. coli.
