Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl.
Gahne et al.
(1987) observed genetic polymorphism of A1B using one-dimensional horizontal polyacrylamide gel electrophoresis followed by Western blotting with specific antiserum.
Three different phenotypes, designated 1-1, 1-2, and 2-2, were observed.
Family data supported the hypothesis that the three phenotypes are determined by 2 codominant alleles at an autosomal locus
In pigs the homologous locus is linked to malignant hyperthermia.
Several other linkages in pigs and in horses suggest that human chromosomes 19, 6, and 1 are 'candidate chromosomes' for bearing the human A1B.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry (frozen): 1:50-500Optimal dilutions to be determined by the researcher.