5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin in the human body.
In chemical analysis of urine samples, 5-HIAA is used to determine the body's levels of serotonin
5-HIAA is tested by 24-hour urine samples combined with an acidic additive to maintain pH below 3.
Certain foods and drugs are known to interfere with the measurement, and levels can vary depending on complications, such as a tumor, renal malfunction, small bowel resection, among other things.
Since 5-HIAA is a metabolite of serotonin, testing is most frequently performed for the diagnosis of carcinoid tumors of the enterochromaffin (Kultschitzsky) cells of the small intestine, which release large amounts of serotonin.
Values greater than 25mg per 24 hours (higher if the patient has malabsorption) are strong evidence for carcinoid.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes 5-HIAA. Species Crossreactivity: pan-species (general)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Immunogen：Small Molecule, 5-HIAA conjugated to OVA.
