84-4404-95　［取扱停止］ZNF76 (Zinc Finger Protein 76, Zinc Finger Protein 523, ZNF76, D6S229E, ZNF523) 50ul　361492

特徴

• ZNF76, also known as ZNF523 or Zfp523, is a transcriptional repressor expressed in the testis.
• It is the human homolog of the Xenopus Staf protein (selenocysteine tRNA gene transcription- activating factor) known to regulate the genes encoding small nuclear RNA and selenocysteine tRNA.
• ZNF76 localizes to the nucleus and exerts an inhibitory function on p53-mediated transactivation.
• ZNF76 specifically targets TFIID (TATA-binding protein).
• The interaction with TFIID occurs through both its N and C termini.
• The transcriptional repression activity of ZNF76 is predominantly regulated by lysine modifications, acetylation and sumoylation.
• ZNF76 is sumoylated by PIAS 1 and is acetylated by p300.
• Acetylation leads to the loss of sumoylation and a weakened TFIID interaction.
• ZNF76 can be deacetylated by HDAC1.
• In addition to lysine modifications, ZNF76 activity is also controlled by splice variants.
• Two isoforms exist due to alternative splicing.
• These isoforms vary in their ability to interact with TFIID.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.