Tryptophanyl-tRNA synthetases are essential enzymes that catalyze the aminoacylation of tRNA(Trp) with tryptophan, an essential function of the cell’s protein synthesis machinery.
Two forms of tryptophanyl-tRNA synthetase exist; a cytoplasmic form, named TrpRS (also known as WARS), and a mitochondrial form, named WARS2.
In normal cells, human TrpRS exists as a full length form and as a truncated form designated mini TrpRS, which is produced by alternative splicing.
Expression of mini TrpRS is highly stimulated in human cells by the addition of IFN-g.
Although both human full length TrpRS and mini TrpRS are enzymatically active in aminoacylation, they differ in angiostatic activity.
The tyrosyl-tRNA synthetase (TyrRS), a protein related to TrpRS, exists as a homodimeric enzyme that catalyzes the aminoacylation of tRNA(Tyr) by L-tyrosine.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human YARS. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human YARS.
