Atrophin interacting proteins (AIP)s bind to atrophin-1 in the vicinity of the polyglutamine tract.
The WW domain consists of aa35-40 and is characterized by four well conserved aromatic residues, two of which are tryptophan.
All five AIPs contain multiple WW domains and can be divided into two distinct classes.
AIP1 and AIP3 (WWP3) are MAGUK-like multidomain proteins containing a guanylate kinase-like region, two WW domains and multiple PDZ domains.
AIP2 (WWP2), AIP4 (itchy) and AIP5 (WWP1) are highly homologous, each having four WW domains and a HECT domain characteristic of ubiquitin ligases.
These interactors are similar to isolated Huntingtin-interacting proteins, suggesting commonality of function between two families of proteins responsible for similar diseases.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human WWP2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human WWP2.
