84-4404-23　［取扱停止］WNK2 (Serine/threonine-Protein Kinase WNK2 (EC:2.7.11.1), Antigen NY-CO-43, Protein Kinase Lysine-deficient 2, Protein Kinase with no Lysine 2, Serologically Defined Colon Cancer Antigen 43, WNK2, KIAA1760, PRKWNK2, SDCCAG43) 50ul　361454

特徴

• The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of aa250-300 organized in two domains.
• WNK, for “with no lysine (K)”, kinases are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydrophobic residues.
• WNK kinases contain a lysine upstream of the traditional position, within a glycine string.
• This lysine functions as an anchor and orients ATP through interactions with the a and ∫ phosphoryl groups.
• The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1.
• The human WNK1 gene encodes a 2382aa protein that is primarily expressed in heart, kidney, muscle and distal nephron.
• The human WNK3 gene encodes a protein that is primarily expressed in brain; the human WNK4 gene encodes a 1243aa protein that is expressed in kidney.
• Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption and impaired K+ and H+ excretion.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.