84-4403-99　［取扱停止］VTI1B (Vesicle Transport through interaction with t-SNAREs Homolog 1B, Vesicle Transport v-SNARE Protein Vti1-like 1, Vti1-rp1, VTI1, VTI1L, VTI1L1, VTI2) 100ul　361442

特徴

• Membrane traffic in eukaryotic cells requires the interaction of a vesicle-associated soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein receptor (v-SNARE) on transport vesicles with a SNARE on the target membrane (t-SNARE).
• Both v- and t-SNAREs are compartment-specific and bind each other directly and specifically.
• The v-SNAREs Ykt6p and Vti1p are involved in ER-Golgi and intra-Golgi membrane trafficking.
• For v-SNARE Ykt6p, membrane interaction is mediated through a cysteine/aliphatic/aliphatic/methionine or histidine (CAAX) C-terminal motif, a consensus sequence involved in prenylated membrane anchoring.
• The v-SNARE Vti1p interacts with the prevacuolar t-SNARE Pep12p in Golgi prevacuolar transport and with the cis-Golgi t-SNARE Sed5p in traffic to the cis-Golgi.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.