Vgl-2 (Transcription cofactor vestigial-like protein 2), also known as VITO-1, is a 317aa protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes.
Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle.
During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus.
Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation.
This evidence suggests that Vgl-2 is essential for muscle gene expression.
There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human VGLL2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa34-79 of human VGLL2.
