The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression.
USP1 (ubiquitin specific peptidase 1), also known as UBP, ubiquitin carboxyl-terminal hydrolase 1, ubiquitin thioesterase 1 or deubiquitinating enzyme 1, is a 785aa that belongs to the peptidase C19 family of ubiquitin C-terminal hydrolases.
A negative regulator of DNA damage repair, USP1 specifically deubiquitinates FANCD2 in the DNA repair pathway.
Following DNA damage, autocatalytic cleavage of USP1 leads to an increase in ubiquitinated PCNA and the recruitment of POL H.
Multiple isoforms of USP1 exist due to alternative splicing events.
Applications:Suitable for use in Western Blot,, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human USP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human USP1.
