Receptor-associated protein 80 (RAP80), also known as Ubiquitin interaction motif-containing protein 1 (UIMC1) or Retinoid X receptor-interacting protein 110 (RXRIP110), is a 719aa protein.
Acting as a transcription repressor, RAP80 interacts with GCNF, thereby blocking GCNF interaction with the corepressor N-CoR.
Localized to the nucleus, RAP80 is phosphorylated upon DNA damage.
RAP80 contains two nuclear localization signals and one UIM (ubiquitin- interacting motif) repeat in the N-terminal, while the C-terminal contains two zinc fingers, a third nuclear localization signal and a potential PEST sequence.
RAP80 is expressed as four isoforms produced by alternative splicing and is present in thymus, testis, heart and ovary.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:200Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human UIMC1.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human UIMC1.
