Glucosylceramide synthase (GCS), also designated ceramide glucosyltransferase, belongs to the glycosyltransferase 2 family.
It is a widely expressed integral membrane protein encoded by UGCG.
The enzyme can be found in the plasma membrane of all eukaryotic cells, and a significant concentration of glucosylceramide synthase activity has been reported in the Golgi complex.
Glucosylceramide synthase catalyzes the first glycosylation step in glycosphingolipid biosynthesis and functions as a glucosyltransferase and flippase in the transfer of glucose to ceramide.
Glucosylceramide synthase operates in cell recognition, cell proliferation and differentiation, immune recognition and signal transduction.
The regulation of ceramide levels through glucosylceramide synthase has been associated with the induction of apoptosis and notable research implicates this relationship with drug-induced apoptosis in a variety of cell types.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human UGCG. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human UGCG.
