Various hormones are secreted from the anterior pituitary during development and growth, including thyroid-stimulating hormone (TSH, also known as thyrotropin), follicle-stimulating hormone (FSH) and leutinizing hormone (LH).
TSH, FSH, and LH are heterodimers formed from a common a chain and a unique Bchain.
TSH is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones.
TSH is regulated by thyroid hormone (T3) and various retinoid compounds.
TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function.
The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation.
In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human TSHR. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human TSHR.
