TREX-1 (three prime repair exonuclease 1), also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS or DNase III, is a member of the exonuclease superfamily and belongs to the TREX family.
Members of the TREX family are involved in DNA metabolism and repair.
TREX-1 is expressed in spleen, liver, thymus, colon, heart, brain and small intestine.
It localizes to the nucleus and exists as a homodimer.
TREX-1 is an exonuclease and its activity requires magnesium as a cofactor.
TREX-1 has a preference for double stranded DNA and functions in the 3’-5’ direction yielding nucleoside 5’-phosphates.
TREX-1 also interacts with the SET complex (an endoplasmic reticulum (ER)-associated complex) and participates in granzyme A-mediated apoptosis.
Mutations or defects in the gene encoding TREX-1 have been associated with a variety of diseases, including systemic lupus erythematosus, chilblain lupus (CHBL), Aicardi-Goutieres syndrome type 1 (AGS1) and type 5 (AGS5) and autosomal dominant retinal vasculopathy with cerebral leukodystrophy (CRV).
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human TREX1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human TREX1.
