The TSG6 gene is transcribed in normal fibroblasts and activated by binding of the cytokines TNFalpha and IL-1 at AP-1 and NF-IL6 sites in its promoter.
TSG-6 is a glycoprotein and a member of the hyaluronan-binding protein family, which includes cartilage link protein, proteoglycan core protein and the adhesion receptor CD44.
TSG-6 is highly homologous to CD44, particularly in the hyaluronic acid-binding domain.
TSG-6 is found in TNF-treated cells; its expression is rapidly activated by TNFalpha, IL-1 and lipopolysaccharide in normal fibroblasts, peripheral blood mononuclear cells, synovial cells and chondrocytes.
The presence of TSG-6 in synovial fluid suggests a possible role in rheumatoid arthritis.
TSG-6 forms a stable complex with components of the serine protease inhibitor, inter-alpha-inhibitor (IalphaI).
TSG-6 potentiates the inhibitory effect of lalphal on the protease activity of plasmin.
Through their cooperative inhibitory effect on plasmin, TSG-6 and lalphal can modulate the protease network and thus inhibit inflammation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human TNFAIP6. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human TNFAIP6.
