84-4401-75　［取扱停止］TFEB (Transcription Factor EB, Class E Basic Helix-loop-helix Protein 35, bHLHe35, BHLHE35) 100ul　361298

特徴

• The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region.
• These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family.
• TFE3 is ubiquitously expressed and can directly associate with DNA as either a homodimer or a heterodimer formed with two related proteins, TFEB or TFEC.
• TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B cell-specific gene transcription and DNA recombination.
• TFEB binds to the major late promoter of adenovirus and specifically associates with DNA as both a homodimer and a heterodimer with TFE3.
• TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta.
• TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation.
• Unlike TFE3, the expression of TFEC is largely restricted to fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.