The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB.
Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB.
IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on the sites that trigger its degradation, 32-36aa.
The functional IKK complex contains three subunits, IKKa, IKKB and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues.
TBK1 was shown to complex with TRAF2 and TANK in the NFkB activation pathway.
TBK1 shares homology with IKKa and IKKB in the N-terminal half, which includes the kinase domain.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human TBK1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human TBK1.
