GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form.
TBC1D2 (TBC1 domain family member 2), also known as Armus or PARIS-1 (prostate antigen recognized and identified by SEREX 1), is a 928aa protein containing a Rab-GAP TBC domain and a PH domain.
Localizing to cytoplasm, cytoplasmic vesicles and cell junctions, TBC1D2 is expressed in a broad range of tissues, including kidney, liver, lung and placenta, as well as in keratinocytes and epithelia-containing organs.
TBC1D2 functions as a GTPase-activating protein for RAB7, and also acts as a linker between RAB7 and RAC1, which leads to RAB7A inactivation and inhibition of E-cadherin degradation.
Existing as six alternatively spliced isoforms, the gene encoding TBC1D2 maps to human chromosome 9q22.33.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide
Specificity：Recognizes human TBC1D2 at ~105kD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues of human TBC1D2.
