TFIID is a general transcription factor that facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element.
TFIID is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs).
The TAF II family members include p18, p28, p32, p100, p130, p170 and p250, which is the largest subunit of TFIID.
TAF II p32 is the human homologue of the Drosophila TAFII40 and is upregulated during apoptosis.
TAFII p32 interacts with the activation domain of the viral protein 16, TFIIB and the class II transactivator (CIITA) to modulate transcription.
The human and murine TAFII p32 proteins are distinct isoforms, designated TAF II p32 a and b, respectively, and are thought to have individual roles in regulation.
TAF II p28 and TAF II p18 interact with one another in vitro and intracellularly, and both interact with TBP through distinct domains.
TAF II p28 potentiates transactivation of the estrogen and vitamin D3 receptors (ER and VDR), and is the limiting factor in the RXRa activation pathway.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human TAF13. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human TAF13.
