SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans.
Nuclear SPT4 and SPT5 are involved in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat.
SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation.
However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation.
The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human SUPT4H1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human SUPT4H1.
