ST8Sia IV (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase IV), also known as PST, PST1 or SIAT8D, is a 359aa single-pass type II membrane protein that localizes to the membrane of the Golgi apparatus.
Highly expressed in heart, thymus and spleen, as well as fetal lung, brain and kidney, ST8Sia IV functions to catalyze the polycondensation of alpha-2,8-linked sialic acid, an event that is required for the synthesis of polysialic acid (PSA).
PSA is an important regulator of neuronal plasticity and is present in embryonic brain tissue, where it interacts with NCAM (neural cell adhesion molecule) and plays a crucial role in fetal brain development.
Defects in the gene encoding ST8Sia IV are associated with idiopathic pancreatitis, schizophrenia and tumor formation/metastasis.
ST8Sia IV exists as multiple isoforms produced by alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide,50% glycerol.
Specificity：Recognizes human ST8SIA4. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human ST8SIA4 .
