Cell type-specific expression of unique carbohydrate structures on cell surface glycoproteins and glycolipids provides information relevant to cell-cell interactions in developing and adult organisms.
Sialyltransferases contribute to the diversity of carbohydrate structures through their attachment of sialic acid in various terminal positions on glycolipid and on glycoprotein (N-linked and O-linked) carbohydrate groups.
The N-acetyllactosaminide alpha 2,3 sialyltransferase (ST3GAL-III), also known as SIAT6 and ST3GAL3, is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates.
Localized to the Golgi apparatus and the Golgi stack membrane, St3Gal-III is secreted into the body fluid.
Twenty-six named isoforms of St3Gal-III exist as a result of alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:20000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ST3GAL3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ST3GAL3.
