84-4400-77　［取扱停止］SRP19 (Signal Recognition Particle 19kD Protein) 50ul　361240

特徴

• Signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain that contains six proteins.
• The S domain contains unique sequence SRP RNA and four SRP proteins: SRP19, SRP54, SRP68 and SRP72.
• The Alu domain contains two SRP proteins, SRP9 and SRP14.
• SRP interacts with ribosomes to bring translating membrane and secreted proteins to the endoplasmic reticulum (ER) for proper processing.
• SRP9 and SRP14 form a heterodimer before binding to SRP RNA, and SRP19 functions in the assembly of SRP and binds to free SRP RNA.
• This event is a prerequisite for the subsequent binding of SRP54 to helix 8 of SRP RNA in eukaryotes and involves an SRP19-induced conformational change in the RNA.SRP54 interacts with both the nascent signal peptide and SRP RNA.
• SRP68 binding to SRP RNA enhances SRP72 binding.
• SRP19, SRP68 and SRP72 are localized in the nucleolus and cytoplasm, whereas SRP54 is only localized in the cytoplasm.
• SRP68 also accumulates in the ER.
• Thus, the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.