84-4400-53　［取扱停止］SPHK2 (Sphingosine Kinase 2, SK 2, SPK 2) 50ul　361228

特徴

• Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P).
• SPP is a bioactive lipid that exerts multiple biological effects in a large variety of cell types, acting as either an intracellular messenger or an extracellular ligand coupled to Edg-family receptors.
• Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors.
• One potent inhibitor of SphK1 activity is DMS (N, N-dimethylsphingosine).
• SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival.
• Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFa.
• Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases.
• SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.
• SphK1 is highly homologous with SphK2, another member of a growing class of sphingolipid kinases.
• Expression of SphK2 mRNA exhibits a markedly different tissue distribution than that of SphK1 and appears at a later stage in embryonic development.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.