L-amino acid transporter protein-2 (LAT2), a non-glycosylated membrane protein, complexes with CD98 to contribute to readsorption of neutral amino acids in renal epithelia and blood-tissue barriers.
The gene encoding LAT2 is expressed primarily in the kidney, but also to a lesser extent in placenta, brain, liver, spleen, skeletal muscle, heart, small intestine, and lung.
Transfection with the antisense sequence of LAT2 suggests that LAT2 expression plays a major role in net basolateral efflux of cysteine, and points to LAT2 as a candidate gene to modulate cysteine reabsorption.
In addition, the CD98/LAT2 heterodimer associates with Integrin ∫1 in intestinal epithelial cells, where ligand binding to CD98 and another cell surface molecule, ICAM-1 differentially regulates LAT2 activity, suggesting a novel mechanism by which events like cell adhesion may affect amino acid transport activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human SLC7A8. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human SLC7A8.
