SETD6 (SET domain-containing protein 6) is a 473aa protein that belongs to the SETD6 family.
Containing one SET domain, SETD6 is structurally similar to the Rubisco large subunit methyltransferase.
SETD6 is a proteinlysine N-methyltransferase that specifically monomethylates “Lys-310” of the NFkB p65 subunit of NFkB complex, leading to down-regulate NFkB transcription factor activity.
SETD6-mediated methylation renders NFkB p65 inert and attenuates NFkB p65-driven transcriptional programs, including inflammatory responses in primary immune cells.
The SETD6-initiated lysine-methylation signaling cascade acts to restrain activation of NFkB-mediated inflammatory responses in diverse cell types.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human SETD6. Species Crossreactivity: mose and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human SETD6.
