Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins.
Among the proteins mediating metastasis are serine proteases, such as neutrophil elastase.
In 1985, Dr.
Jim Travis and Dr.
R.W.
Carrell designated an emerging family of serine protease inhibitors as the serpin family, which share homology in both primary aa sequence and tertiary structure.
Serpins contain a stretch of peptide that mimics a true substrate for a corresponding serine protease.
Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive.
Aberrant expression of serpin family members can contribute to a number of conditions, including emphysema (a-1 antitrypsin deficiency), fatal bleeding (elastase to thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers).
Human chromosome position 18q21.3 contains a cluster of serpins, including a tandem duplication of the SCCA gene, plasminogen activator inhibitor type 2, and maspin.
SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1.
The human SCCA1 gene encodes a 390aa protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human SERPINB3/4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human SERPINB3/4.
