SUMO (small ubiquitin-related modifier), a member of the ubiquitin-like protein family, regulates diverse cellular functions of a variety of target proteins, including transcription, DNA repair, nucleocytoplasmic trafficking and chromosome segregation.
SUMO precursor proteins undergo cleavage of the residues after the “GG” region by SUMO-specific proteases in maturation.
This cleavage of the precursor is a prerequisite for subsequent sumoylation.
The sentrin-specific (or SUMO-specific) protease (SENP) proteins belong to the peptidase C48 family and include SENP1-3 and SENP5-8.
SENP1, SENP2 and SENP3 degrade UBL1 and SMT3H2 conjugates and subsequently release the monomers from sumoylated substrates.
HIPK2 is a desumoylation target for SENP1 which shuttles between the cytoplasm and the nucleus.
Mutation analyses reveal that SENP1 contains the nuclear export sequence (NES) within the extreme carboxyl-terminal region, and SENP1 is exported to the cytoplasm in a NES-dependent manner.
SENP2 has been implicated as a downregulator of CTNNB1 levels and may therefore be a modulator of the Wnt pathway.
SUMO protease SENP3 reverses the sumoylation of MEF2 to augment its transcriptional and myogenic activities.
SENP5 localizes to the nucleolus and preferentially processes SUMO-3.
It is thought to play a role in mitosis and/or cytokinesis.
SENP6 localizes to the cytoplasm and releases SUMO-1.
Expression of SENP6 is higher in reproductive organs, indicating that it may mediate processes related to reproduction.
SENP8 is involved in the release of sentrins.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human SENP6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human SENP6.
