Stearoyl-CoA desaturase (SCD) is a microsomal enzyme required for the synthesis of oleate and palmitoleate, which are the major monounsaturated fatty acids of membrane phospholipids, triglycerides and cholesterol esters.
SCD plays a major role in the triacylglycerol and phospholipid secretion process and in mechanisms of cellular cholesterol homeostasis.
It is subject to rapid turnover in the cell and, as such, represents a model for studying selective degradation of short-lived proteins of the ER.
SCD is also an important regulator of membrane fluidity.
An increase in expression levels of SCD is observed in cells which are induced to differentiate into adipocytes and in certain tumor cell lines.
Due to gene duplication events, the number of genes in the SCD family differs between species.
Their expression patterns are affected by the level of unsaturated fatty acids in the diet of the animal.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human SCD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human SCD.
