Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins.
Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions.
Ribosomal Protein S14, also known as RPS14 or EMTB, is a 151aa component of the small ribosomal 40S subunit.
Localized to the cytoplasm, Ribosomal Protein S14 is a member of the S11P family of ribosomal proteins and is highly conserved among several species.
Defects in the gene encoding Ribosomal Protein S14 may cause resistance to emetine, a protein synthesis inhibitor found in Chinese hamster ovary cells.
Multiple isoforms of this protein exist due to alternative splicing events.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RPS14. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human RPS14.
