The C-terminal of rabphilin-3A consists of two C2 domains, A and B, and the N-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs.
Rabphilin-3A belongs to a family of other C-terminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2.
Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules.
Rabphilin-3A binds Rab3a/GTP/Mg+ within N-terminal residues 45 and 170.
Rabphilin-3A binds calcium ions and phosphatidylinositol 4, 5-bisphophate containing lipid vesicles within its C2 domains.
Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis.
Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other.
Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RPH3A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human RPH3A.
