The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses.
Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB that is composed of three subunits, designated RPA 14kD (also known as RPA3), RPA 32kD and RPA 70kD.
Together, these subunits play an important role in DNA replication, recombination and repair.
RPA is one of the major damage-recognition structures involved in the early stage of nucleotide excision repair and may play a role in telomere maintenance.
The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5’ side of a ssDNA substrate and then extends in the 3’ direction to create a stably bound hRPA.
The RPA 14kD subunit localizes to the nucleus and is the smallest component of the RPA complex, functioning with the other subunits to regulate various aspects of DNA metabolism.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RPA3.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human RPA3.
