84-4398-06　［取扱停止］RGS7 (Regulator of G-Protein Signaling 7) 100ul　361091

特徴

• Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors.
• G proteins comprise a, Band g subunits, and following activation the α subunit binds GTP and dissociates from the Bg complex.
• A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Ga isoforms by increasing the rate at which they convert GTP to GDP.
• A subfamily of RGS proteins expressed in the central nervous system contain, in addition to the highly conserved RGS domain, a characteristic GGL domain, or G protein g subunit-like domain, which mediates binding to GB 5 subunits.
• This subfamily, which includes RGS6, RGS7, RGS9 and RGS11, associates with GB 5 to form active GAP complexes that are predominantly localized to the cytosol.
• RGS/B5 complexes preferentially target Gao subunit for hydrolysis and inhibit GB1g2-mediated activation of phospholipase C.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.