84-4398-03　［取扱停止］RGS3 (Regulator of G-Protein Signaling 3, RGP3) 50ul　361090

特徴

• Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors.
• G proteins comprise a, b and g subunits, and following activation, the a subunit binds GTP and dissociates from the bg complex.
• A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Ga isoforms by increasing the rate at which they convert GTP to GDP.
• RGS3 is a protein of the RGS family that preferentially binds to the activated form of Ga11.
• Through this association, RGS3 inhibits Ga11-induced signaling, leading to a decrease in the accumulation of intracellular calcium and the inhibition of MAP kinase phosphorylation.
• RGS3 is highly expressed in adult kidney and myocardium, and it is primarily localized to the cytoplasm.
• Upon activation of Ga11, RGS3 translocates from the cytosol to the plasma membrane, thereby bringing RGS3 within close proximity to the targeted G protein.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.