RECK (reversion-inducing-cysteine-rich protein with Kazal motifs) is a membrane anchored glycoprotein that binds to and inhibits the proteolytic activity of matrix metalloproteinase-9 (MMP-9).
The enzymatic activity of MMP-9 facilitates tumor invasion by proteolytically digesting the extracellular matrix, thereby enabling tumor growth, expansion and metastasis.
RECK inhibits the secretion and activation of MMP-9 into the extracellular matrix, which results in the inhibition of tumor growth.
RECK contains multiple EGF-like repeats and serine-protease inhibitor-like domains.
The expression of RECK is suppressed in several tumors and oncogenically transformed cells, suggesting that the loss of RECK activity correlates with transformed phenotypes.
Transcriptional activation of RECK is potentially negatively regulated by the Sp1 family of transcription factors, as it contains two Sp1 binding motifs in the promoter region, and, in cells transformed with the ras oncogene, the Sp1 promoter region is essential for repressing RECK gene expression.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RECK. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human RECK.
