Rb (retinoblastoma protein) is a potent transcriptional regulator that is directly involved with events such as entry into cell division and formation of heterochromatin.
RBP2 (Retinoblastoma-binding protein 2), also known as RBBP2, JARID1A (Jumonji/ARID domain-containing protein 1A) or KDM5A, is a nuclear protein that belongs to the JARID1 histone demethylase family.
Expressed ubiquitously, RBP2 functions as a histone demethylase that, in conjunction with other proteins, binds directly to the viral-binding domain of Rb, thereby regulating Rb-mediated cell proliferation events.
In addition, RBP2 can bind to the Rb-interacting protein rhombotin-2 (LMO2) and, through this interaction, can indirectly modulate Rb activity.
Via its demethylase activity, RBP2 can remove methyl residues from Histone H3, thus playing a crucial role in the histone code.
RBP2 contains one ARID domain, three PHD-type zinc fingers, one JMJN domain and one JMJC domain through which it conveys its enzymatic activity.
Multiple isoforms of RBP2 exist due to alternative splicing events.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:200Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RBP2.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human RBP2.
