The human retinoblastoma gene product plays an important role in the negative regulation of cell proliferation.
Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins.
pRb and the structurally related p107 form complexes with E2F, a transcription factor originally identified through its role in transcriptional activation of the adenovirus E2 promoter.
Moreover, pRB and p107 share a high degree of structural homology in the adenovirus E1A binding domain (i.e.
“pocket region”) that is believed to play a primary role in the function of these proteins.
A protein designated p130 shows a high degree of identity with pRb and p107 and also possesses a pocket region.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human RBL1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human RBL1.
