RAIN, also known as RASIP1 (Ras-interacting protein 1), is a 963aa protein that contains one dilute domain and one Ras-associating domain.
The RAIN protein is highly expressed in heart and detected at lower levels in placenta and pancreas.
RAIN interacts with H-Ras, Rap 1A, Rap 2, R-Ras, Raf-1 and TC 21, as well as with Ras family members that have been activated by GTP binding.
RAIN interacts with Ras in a GTP-dependent manner in vitro and in vivo, requiring an intact Ras core effector-binding domain for this interaction, and thus fits the definition of a Ras effector.
Unlike other Ras effectors, RAIN localizes to the perinuclear, juxta-Golgi vesicles in intact cells and is recruited to the Golgi by activated Ras.
Containing twelve exons, the RAIN gene is conserved in dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 19q13.33.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human RASIP1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to residues in human RASIP1.
