84-4397-60　［取扱停止］RAPSN (43kD Receptor-Associated Protein of the Synapse, RAPsyn, 43kD postsynaptic Protein, Acetylcholine Receptor-Associated 43kD Protein, RING Finger Protein 205, RNF205) 50ul　361066

特徴

• The RAPSN gene locus is located on chromosome 11p11.1-p11.2 and encodes a peripheral membrane protein.
• Rapsyn (receptor-associated protein of the synapse) is expressed in the postsynaptic membrane of skeletal muscle.
• Rap-syn is required for the clustering of nicotinic acetylcholine receptors (nAChR).
• Rapsyn self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs).
• Rapsyn interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain.
• Rapsyn modifies trafficking of AChR within the cell.
• Expression is essential for agrin-induced AChR clustering.
• Overexpression inhibits agrin-induced AChR clustering pathway.
• Absence of rapsyn causes deficit in the formation of postsynaptic specializations at neuromuscular synapse, which increases axonal branching and motonueron survival.
• Rapsyn plays a role in selective targeting of newly synthesized intracellular AChR to postsynaptic membrane.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.